We now know all of the genes in an organism, but we do not know the function of many of them (usually >50% of the predicted genes in eukaryotic genomes) and … advantages of 2-D protein electrophoresis and Proteomics +can identify virulence factors differentially regulated at transciptional or postranscriptional levels It appears, however, that the prospective advantages surpass the threats. Ideally, over the next year approximately, more medical researches will be done on individuals, and we'll be more sure of the advantages and disadvantages of resveratrol (and able making a more educated choice). Advantages and disadvantages with transposable elements? Disadvantages are that it's difficult to find natural mutants for single nucleotide base changes for example. advantages and disadvantages of using Classical factionation ... genes can then be identified by reverse genetics. I picked transposable elements for the forward genetics and RNAi as a reverse genetics technique. But with more scientific research and experiments being done with the technology at full swing, it’s a matter of discussion everywhere. ... Tab l e 4. 3. These reverse genetics resources allow for the identification of mutations in candidate genes and subsequent phenotypic analysis of these mutants. Advantages are that it is easy to find a broad area that causes the phenotype. PCR – principle, advantages and disadvantages Posted by Dr. Traian Stefan Grozescu on March 31, 2013 October 5, 2013 Developed in 1983 by Kary Mullis , [1] PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. Reverse genetics – gene -> mutant phenotype In the post-genomic era, the classical problem has been reversed. Hence, the word “reverse” is used. Reverse transfection is a technique for the transfer of genetic material into cells.As DNA is printed on a glass slide for the transfection process (the deliberate introduction of nucleic acids into cells) to occur before the addition of adherent cells, the order of addition of DNA and adherent cells is reverse that of conventional transfection. All over the world, scientists keep discussing the opportunities and risks involved in genome editing. Just a decade back, Gene editing was not even a term. - (Oct/22/2007 ) Having some trouble with a question where you should describe a forward and a reverse genetics technique briefly.